flowAI report
SIgN_GM
FCS file information
Input file name: Donor_Aorta-Blood_023
Number of events: 1157223
Quality control analysis
Summary
The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 57.9 %
Number of high quality events: 487140
Flow rate check
24.54 % anomalies detected in the flow rate check.
The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.
Signals acquisition check
55.84 % anomalies detected in the signal aquisition check.
The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.
The FCS file was divided in 2315 bins, hence each bin contains 500 event(s). The stable region is located between the bins 109 and 1130.
More info on the changepoints detected
Changepoints detected in channels with shifts in signal acquisition:
## 1
## 488 525/50-A 1643
## 488 715/30-A 1459
## 405 515/20-A 1808
## 405 605/40-A 1241
## 405 670/30-A 1173
## 405 780/60-A 1974
## 355 379/28-A 109
## 355 560/40-A 1132
## 355 740/35-A 1982
## 355 820/60-A 1837
## 640 780/60-A 1947
## 561 585/15-A 1143
## 561 610/20-A 1327
## 561 780/60-A 1130
## 405 585/15-A 1647Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.
No changepoints were detected in the channels: 405 450/50-A, 405 710/40-A, 355 515/30-A, 640 670/30-A, 640 730/35-A.
Dynamic range check
1.05 % anomalies detected in dynamic range check.
The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.
More info on the anomalies detected in the dynamic range check
For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:
## lower_range upper_range
## 488 525/50-A 141 2334
## 488 715/30-A 4 53
## 405 450/50-A 3 96
## 405 515/20-A 6 2602
## 405 605/40-A 0 116
## 405 670/30-A 6 97
## 405 710/40-A 6 77
## 405 780/60-A 232 21
## 355 379/28-A 54 238
## 355 515/30-A 13 16
## 355 560/40-A 9 242
## 355 740/35-A 5 30
## 355 820/60-A 657 5
## 640 670/30-A 62 18
## 640 730/35-A 167 12
## 640 780/60-A 254 16
## 561 585/15-A 5 7779
## 561 610/20-A 4 2908
## 561 780/60-A 5 39
## 405 585/15-A 3 117
## total_SUM 1636 16816
## total_UNIQUE 1259 10886Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.